Coding

Part:BBa_K1688017:Design

Designed by: Mikael Andersson Schönn   Group: iGEM15_Uppsala   (2015-09-15)


Nah7 - Mutagenized upper naphthalene breakdown pathway (inc RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4139
    Illegal NotI site found at 8182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5232
    Illegal BamHI site found at 3217
    Illegal BamHI site found at 5919
    Illegal XhoI site found at 1328
    Illegal XhoI site found at 3716
    Illegal XhoI site found at 3868
    Illegal XhoI site found at 9370
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1821
    Illegal NgoMIV site found at 3596
    Illegal NgoMIV site found at 5470
    Illegal NgoMIV site found at 6082
    Illegal NgoMIV site found at 9395
    Illegal AgeI site found at 659
    Illegal AgeI site found at 3590
    Illegal AgeI site found at 6298
    Illegal AgeI site found at 8293
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2938
    Illegal BsaI site found at 3611
    Illegal BsaI site found at 4202
    Illegal BsaI site found at 5368
    Illegal BsaI.rc site found at 2772
    Illegal BsaI.rc site found at 5522
    Illegal BsaI.rc site found at 7807
    Illegal SapI.rc site found at 3302
    Illegal SapI.rc site found at 3848
    Illegal SapI.rc site found at 7699


Design Notes

The pathway contained two endogenous restriction sites for the enzyme PstI which have been removed through site directed mutagenesis.


Source

Nah7 plasmid from the PpG7 strain of Pseudomonas putida. Standard iGEM registry RBS.

References